نشریه محیط زیست طبیعی

بررسی تأثیر حفاظتی EGCG بر صدمات کروموزومی ناشی از تیمار با نیکل بر ردة سلولی HFF انسانی با استفاده از آزمون میکرونوکلئوس در سلول‌های دو هسته‌ای

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست‌شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد مشهد، مشهد، ایران.

2 گروه زیست شناسی، دانشکدة علوم، دانشگاه فردوسی مشهد، مشهد، ایران.

10.22059/jne.2023.350599.2487

چکیده

نیکل از جمله فلزات سنگینی است که به‌دلیل کاربرد وسیع در صنایع به‌طور گسترده در طبیعت پیرامون ما یافت می‌شود. صدمات کروموزومی ناشی از نیکل به توانایی آن در ایجاد گونه‌های فعال اکسیژن نسبت داده می‌شود. امروزه استفاده از ترکیبات گیاهی جهت جلوگیری از صدمات کروموزومی ناشی از این عوامل به‌طور گسترده‌ای رایج شده است. چای سبز با داشتن انواعی از ترکیبات پلی‌فنول خصوصاً اپیگالوکاتچینگالات (EGCG) که می‌تواند به‌عنوان یک نوشیدنی رایج استفادة روزمره داشته باشد، گزینة مناسبی برای محافظت در برابر فلزات سنگین از جمله نیکل است. در این تحقیق تأثیر سه غلظت 10، 20 و 30 میلی‌مولار EGCG بر صدمات کروموزومی ناشی از تیمار ردة سلولی  HFFبا غلظت‌های 0/2، 0/5 و 0/7 میلی‌مولار از نیکل با کمک آزمون میکرونوکلئوس در سلول‌های دو هسته‌ای مورد بررسی قرار گرفت. در غلظت‌های مختلف، نیکل توانست فراوانی سلول‌های دو هسته‌ای دارای میکرونوکلئوس که بیانگر صدمات کروموزومی هستند، را به‌شکل معنی‌داری نسبت به تیمار کنترل افزایش دهد. در حالی‌که، در تمام غلظت‌های ذکر شده، EGCG توانست به شکل وابسته به غلظت، باعث کاهش فراوانی این سلول‌ها گردد. نتایج نشان داد که EGCG قادر به کاهش اثرات کلاستوژنتیک نیکل در شرایط برون تنی است. در غلظت‌های بالاتر این توانایی افزایش می‌یابد. با توجه به توانایی آنتی‌اکسیدانی EGCG می‌توان نتیجه گرفت که نیکل بیشتر صدمات کروموزومی خود را با افزایش میزان گونه‌های فعال اکسیژن القاء می‌کند.

کلیدواژه‌ها

عنوان مقاله [English]

Analysis of the protective effect of EGCG against chromosomal damages induced by Nickel treatment of HFF cell line using micronucleus assay on binucleated cells

نویسندگان [English]

  • Niknaz Shahraki 1
  • Farhang Haddad 2

1 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

2 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

چکیده [English]

Nickel is a heavy metal, which due to its wide use in industries, found all around our habitat. Nickel-induced chromosomal abnormalities are related to its ability in producing reactive oxygen species (ROS). Use of herbal ingredients in preventing chromosomal abnormalities induced by such elements has been become widely common. Green tea with its polyphenols especially Epigallocatechin gallate (EGCG) which could be used as a common drink is a suitable choice for protecting against heavy metals including Nickel. In this study, effect of three doses of 10, 20 and 30 mM of EGCG on chromosomal abnormalities induced by 0.2, 0.5 and 0.7 mM of Nickel in HFF cell line was investigated using micronucleus assay on Binucleated cells. In different doses, Nickel was able to increase the frequency of Micronucleated Binucleates (MnBi) significantly, which represents chromosomal abnormalities, in comparison to control. While, in all doses mentioned earlier, EGCG was able to dose dependently decrease the frequency of this cells. The results showed that EGCG is able to reduce clastogenic effect of Nickel in vitro. This ability increases in higher doses. Considering the antioxidant ability of EGCG, it could be concluded that Nickel induces most of its chromosomal abnormalities by increasing ROS.

کلیدواژه‌ها [English]

  • Nickel
  • HFF cell line
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نشریه محیط زیست طبیعی
دوره 76، شماره 3
مهر 1402
صفحه 391-398