Molecular identification of mucoralean fungi isolated from the oil-polluted sites
Document Type : Research Paper
Authors
1 Assistant Professor, Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
2 Professor, Department of Parasitology, Mashhad University of Medical Science, Mashhad, Iran
3 Ahvaz Jondishapur University of Medical Sciences
Abstract
Mucoralean fungi are a group of basal fungi related to zygomycetes which can be found in different environments all over the world. They are a part of fermented food production, secondary metabolites and enzymes. Considering their fast growth and their ability to produce a different sort of enzymes, we seek for their role in oil-polluted sites’ bioremediation. Using oil floating method, fungal isolates were collected and identified both morphologically and molecularly using ITS gene as a barcoding marker and phylogenetic tree. In total, 131 isolates were collected. Among them, 27 mucoralean fungi were listed as follows: Rhizopus (n=19), Mucor (n=4), Actinomucor (n=2), Lichtheimia, and Cunninghamella one isolate per each. These isolated strains from Iran were identified correctly based on molecular methods and then deposited in the culture collections of the Netherlands (Westerdijk Institute) and Warsaw University. Being isolated from oil-polluted environments, these isolates have bioremediation potentials.
Keywords
Abe, A., Oda, Y., Asano, K., Sone, T., 2007. Rhizopus delemar is the proper name for Rhizopus oryzae fumaric-malic acid producers. Mycologia 99, 714-722.
Adeli, H.R., Zare, K., Naimi, N., Alamdar, M., 2016. Changes in lung tissue and white blood cells due to toluene. Journal of Jahrom University of Medical Sciences 14, 1
Atagana, H.I., 2003. Bioremediation of creosote-contaminated soil: a pilot-scale land farming evaluation. World Journal of Microbiololigal Biotechnology 19, 571-581
Badali, H., Prenafeta-Boldu, F.X., Guarro, J., Klaassen, C.H., Meis, J.F., de Hoog, G.S., 2011. Cladophialophora psammophila, a novel species of Chaetothyriales with a potential use in the bioremediation of volatile aromatic hydrocarbons. Fungal Biology 115(10), 1019-29.
Battaglia, P., Andaloro, F., Esposito, V., Granata, A., Guglielmo, L., Guglielmo, R., Musolino, S., Romeo, T., Zagami, G., 2016. Diet and trophic ecology of the lanternfish Electrona risso (Cocco 1829) in the Strait of Messina (central Mediterranean Sea) and potential resource utilization from the Deep Scattering Layer (DSL). Journal of Marine Systems 159:100-8.
Cox, H.H.J., 1995. Styrene removal from waste gas by the fungus Exophiala jeanselmei in a biofilter. PhD Thesis, University of Groningen, Groningen, the Netherland
Cutright, T.J., 1995. Polycyclic aromatic hydrocarbon biodegradation and kinetics using Cunninghamella echinulata var. elegans. International Biodeterioration & Biodegradation 35, 397-408.
de Hoog, G.S., Yurlova, N.A., 1994. Conidiogenesis, nutritional physiology and taxonomy of Aureobasidium and Hormonema. Antonie van Leeuwenhoek 65(1), 41-54.
de Hoog, G.S., van den Ende, A.G., 1998. Molecular diagnostics of clinical strains of filamentous Basidiomycetes: Molekulare Diagnostik klinischer Stämme filamentöser Basidiomyzeten. Mycoses 41(5‐6), 183-9.
de Hoog, G.S., Zeng, J.S., Harrak, M.J., 2006. Exophiala xenobiotica sp. nov., an opportunistic black yeast inhabiting environments rich in hydrocarbons. Antonie van Leeuwenhoek 90, 257-268.
de Hoog, G.S., Guarro, J., Gené J., Figueras, M.J., 2009. Atlas of Clinical Fungi. Centraalbureau voor Schimmelcultures, 1124 pp., Utrecht/Reus.
de Mello, J.M.M., de Lima Brandão, H., de Souza, A.A.U., da Silva, A., Ulson, S.M., 2010. Biodegradation of BTEX compounds in a biofilm reactor-modeling and simulation. Journal of Petroleum Science and Engineering 70(1-2), 131-139.
Dias, M.A., Lacerda, I.C., Pimentel, P.F., deCastro, H.F., Rosa, C.A. 2002. Removal of heavy metals by an Aspergillus terreus strain immobilized in polyurethane matrix. Letters in Applied Microbiology 34(1), 46-50.
Dolatabadi, S., de Hoog, G. S., Meis, J. F, Walther, G., 2014a. Species boundaries and nomenclature of Rhizopus arrhizus (syn. R. oryzae). Mycoses 57(s3), 108-127.
Dolatabadi, S., Walther, G., Gerrits, A.H.G., de Hoog, G.S., 2014b. Diversity and delimitation of Rhizopus microsporus. Fungal Divers 64: 145-163
Dolatabadi, S., 2015. Mucorales between food and infection. Universiteit van Amsterdam
Dolatabadi, S., Scherlach, K., Figge, M., Hertweck, C., Dijksterhuis, J., Menken, S.B., de Hoog, G.S. 2016. Food preparation with mucoralean fungi: A potential biosafety issue?. Fungal Biology 120(3), 393-401.
Dolatabadi, S., Rezaei-Matehkolaei, A., Pawlowska, J., Hosseini, S.A., Najafzadeh, M.J., Madrid, H., 2019. Chaetothyrialean fungi from aromatic hydrocarbon polluted environments of Iran. Nova Hedwigia 108(3-4), 405-26.
Dos Santos, E.O., Da Rosa, C.F.C., Dos Passos, C.T., Sanzo, A.V.L., De Medeiros Burkert, J.F., Kalil, S.J., Burkert, C.A.V., 2008. Pre-screening of filamentous fungi isolated from a contaminated site in Southern Brazil for bioaugmentation purposes. African Journal of Biotechnology 7, 1314-1317.
Esterhuizen-Londt, M., Schwartz, K., Pflugmacher, S., 2016. Using aquatic fungi for pharmaceutical bioremediation: uptake of acetaminophen by Mucor hiemalis does not result in an enzymatic oxidative stress response. Fungal Biology 120, 1249-1257
Guimarães, L.H.S., Peixoto-Nogueira, S.C., Michelin, M., Rizzatti, A.C.S., Sandrim, V.C., Zanoelo, F.F., Aquino, A.C.M.M., Junior, A.B., Polizeli, M.L.T.M., 2006. Screening of filamentous fungi for production of enzymes of biotechnological interest. Brazilian Journal of Microbiology 37, 474-480
Hermet, A., Méheust, D., Mounier, J., Barbier, G., Jany, J.L. 2012. Molecular systematics within the genus Mucor with special regard to species encountered in cheese. Fung Biol 116: 692–705
Hibbett, D.S., Binder, M., Bischoff, J.F., Blackwell, M., Cannon, P.F., Eriksson, O.E., Huhndorf, S., James, T., Kirk, P.M., Lücking, R., Lumbsch, H.T., 2007. A higher-level phylogenetic classification of the Fungi. Mycological Research 111(5), 509-547
Hölker, U., Bend, J., Pracht, R., Müller, T., Tetsch, L. de Hoog, G.S., 2004. Hortaea acidophila, a new acidophilic black yeast from lignite. Antonie van Leeuwenhoek 86, 287-294
Isola, D., Selbmann, L., de Hoog, G.S., Fenice, M., Onofri, S., Prenafeta-Boldú, F.X., 2013. Isolation and screening of black fungi as degraders of volatile aromatic hydrocarbons. Mycopathologia 175(5-6), 369-379.
Iwatsu, T., Miyaji, M., Okamoto, S. 1981. Isolation of Phialophora verrucosa and Fonsecaea pedrosoi from nature in Japan. Mycopathologia 75, 149-158
Kim, M.J., Lee, H., Choi, Y.S., Kim, G.H., Huh, N.Y., Lee, S., Lim, Y.W., Lee, S.S., Kim, J.J., 2010. Diversity of fungi in creosote-treated crosstie wastes and their resistance to polycyclic aromatic hydrocarbons. Antonie Van Leeuwenhoek 97(4), 377-87.
Krings, M., Taylor, T.N., Dotzler, N., 2013. Fossil evidence of the zygomycetous fungi. Persoonia 30, 1-10.
Krivibok, S., Miriouchkine, E., Seigle-Murandi, F., Benoit-Guyod, J.L., 1998. Biodegradation of Anthracene by Soil Fungi. Chemorphere 31(3), 523-530
Lee, Y.L., Pai, M.C., Chen, J.H., Guo, Y., 2003. Central neurological abnormalities and multiple chemical sensitivity caused by chronic toluene exposure. Occupational Medicine 53(7), 479-82
Leitão, A.L., 2009. Potential of Penicillium Species in the Bioremediation Field. International Journal of Environmental Research and Public Health 6, 1393-1417
Li, P., Sun, T., Stagnitti, F., Zhang, C., Zhang, H., Xiong, X., Allinson, G., Ma, X., Allinson, M., 2002. Field-scale bioremediation of soil contaminated with crude oil. Environmental Engineering Science 19(5), 277-289.
Lors, C., Damidot, D., Ponge, J., Perie, F., 2012. Comparison of a bioremediation process of PAH in a PAH contaminated soil at field and laboratory scales. Environmental Pollution 165, 11-17
Masclaux, F., Guého, E., de Hoog, G.S., Christen, R., 1995. Phylogenetic relationships of human-pathogenic Cladosporium (Xylohypha) species inferred from partial LS rRNA sequences. Journal of Medical and Veterinary Mycology 33(5), 327-38.
Moghimi, H., Hasani Zadeh, P., Hamedi, J., Heidarytabar, R., Azin, E., 2017. Introduction of Fusarium sp. UTMC 5039 as a potent fungal strain for biosurfactant production and evaluation of its potential for crude oil bioremediation. Biological Journal of Microorganism 6(23), 19-33.
Mukherjee, I., Mitta, A., 2005. Bioremediation of Endosulfan using Aspergillus terreus and Cladosporium oxysporum. Bulletin of Environmental Contamination and Toxicology 75, 1034-1040
Pawłowska, J., Okrasińska, A., Kisło, K., Aleksandrzak-Piekarczyk, T., Szatraj, K., Dolatabadi, S., Muszewska, A. 2019. Carbon assimilation profiles of mucoralean fungi show their metabolic versatility. Scientific Reports 9(1), 1-8
Potin, O., Rafin, C., Veignie, E., 2004. Biodegradation of an aged polycyclic aromatic hydrocarbons (PAHs) in contaminated soil by filamentous fungi isolated from the soil. International Biodeterioration & Biodegradation 54, 45-52
Prenafeta Boldú, F.X., de Hoog, G.S., Summerbell, R.C., 2006. Fungi growing on aromatic hydrocarbons: biotechnology's unexpected encounter with biohazard. FEMS Microbiology Review 30: 109-130
Prenafeta-Boldú, F., Ballerstedt, H., Gerritse, J., Grotenhuis, J., 2004. Bioremediation of BTEX hydrocarbons: effect of soil inoculation with the toluene-growing fungus Cladophialophora sp. strain T1. Biodegradation 15(1), 59-65
Purnomo, A.S., Mori, T., Kamei, I., Kondo, R., 2011. Basic studies and applications on bioremediation of DDT: A review. International Biodeterioration & Biodegradation 65(7), 921-930
Qi, B., Moe, W., Kinney, K., 2002. Biodegradation of volatile organic compounds by five fungal species. Applied Microbiology and Biotechnology 58(5), 684-689
Singh, R., Majumdar, R.S.J.R. 2019. Bioinformatics RAJo. Comparative Study on Bioremediation for Oil Spills Using Microbes 4(1), 16-24
Van Groenestijn, J.W., van Heiningen, W.N.M., Kraakman, N.J.R., 2001. Biofilters based on the action of fungi. Water Science Technology 44, 227-232.
Walther, G., Pawłowska, J., Alastruey-Izquierdo, A., Wrzosek, M., Rodriguez-Tudela, J.L., Dolatabadi, S., Chakrabarti, A., de Hoog, G.S., 2013. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30, 11-47
Walther, G., Wagner, L., Kurzai, O., 2019. Updates on the taxonomy of Mucorales with an emphasis on clinically important taxa. Journal of Fungi 5(4), 106.
William, S., Feil, H., Copeland, A., 2012. Bacterial genomic DNA isolation using CTAB. Sigma 50(6876).
Yanto, D.H.Y., Tachibana, S. 2013. Biodegradation of petroleum hydrocarbons by a newly isolated Pestalotiopsis sp. NG007. International Biodeterioration & Biodegradation 85, 438-450
Yazdanparast, S.A., Mohseni, S., de Hoog, G.S., Aslani, N., Sadeh, A., Badali, H., 2017. Consistent high prevalence of Exophiala dermatitidis, a neurotropic opportunist, on railw ay sleepers. Journal de Mycologie Médicale 27(2), 180-187.
)