The efficacy of silver nanoparticles and oxytetracycline in treating Aeromonas hydrophila bacterial infection in common carp (Cyprinus carpio)
Document Type : Research Paper
Authors
Department of Fisheries, Faculty of Natural Resources, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
10.22059/jne.2024.381255.2705
Abstract
One of the most common pathogenic bacteria is Aeromonas hydrophila, which causes various infections with bleeding in farmed fish. The general solution is to use different antibiotics to deal with this bacterium. On the other hand, nanoparticle technology based on antibacterial properties is considered a useful alternative to antibiotics. Therefore, in this study, the effectiveness of 20% oxytetracycline antibiotic and silver nanoparticles with a size of 20-60 nm in curing the disease caused by A. hydrophila bacteria in common carp fish has been considered. For this purpose, 5 treatments were considered: negative control, positive control, patient with oxytetracycline treatment, patient with silver nanoparticle treatment, and patient with combined treatment of oxytetracycline and silver nanoparticle. The consumption amount of oxytetracycline and silver nanoparticles was estimated as 0.36 g and 0.0012 mg, respectively. According to the results, the amount of white blood cells in the positive control group was the highest (20.18±0.76) and different from other treatments (P=0.00, F=122.95). Also, the number of red blood cells (P=0.00, F=18.38) and hemoglobin (P=0.01, F=12.43) in the negative control group was the highest (Hb=8.40±0.79 and RBC=2.40±0.11), and in the positive control group had the lowest value (Hb=5.27±0.60 and RBC=1.38±0.26). The lowest and highest survival percentage was observed in the negative control group (99.00±1.00) and the positive control group (21.33±2.52), respectively. There is a correlation between red blood cell increase, hemoglobin increase, white blood cell decrease, and increased survival. In general, silver nanoparticles and oxytetracycline play an effective role in reducing infectious symptoms and inhibiting bacteria. The simultaneous use of oxytetracycline and nanosilver seems appropriate if it is under-documented and management planning, to reduce treatment costs and environmental risks.
Keywords
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