Investigating the toxicity of zinc oxide nanoparticles compared to its different ions on biochemical parameters and damage to the spleen and testis of male mice
Document Type : Research Paper
Authors
Department of Environmental Engineering, Faculty of Natural Resources and Environment, University of Birjand, Birjand, Iran.
10.22059/jne.2024.378169.2687
Abstract
Despite the wide application of nano materials, there is very little information about the impact of different nanoparticles on human health and the environment. Therefore, the purpose of this study is to investigate the toxicity of zinc oxide nanoparticles (ZnO NPs) compared to its different ions on biochemical parameters and damage to the spleen and testis of male mice. Mice were randomly exposed to ZnO NPs and its various ions in 5 groups of 5 (one control group). After the end of the test period (28 days), blood serum was collected and histological studies of mice spleen and testis were performed. Based on the obtained results, the serum creatinine level of all studied groups increased compared to the control group, while zinc nitrate (Zn (NO3)2) and zinc chloride (ZnCl2) caused a significant increase in the creatinine level (P<0.05) compared to the control group. Also, the level of serum bilirubin exposed to ZnO NPs and zinc sulfate (ZnSO4) did not change compared to the control group, while Zn (NO3)2 and ZnCl2 caused a significant increase (P<0.05) in the level of blood serum bilirubin compared to the control group. Among the histological damages of the mouse testis, we can mention the change in the shape of the spermatogenic tubes, the increase in the number of leydig cells and the decrease in the population of spermatozoid cells and in the spleen tissue, it includes oedema and infiltration of inflammatory cells, arterial congestion, hemosiderin increase and spleen bleeding. Based on the obtained results, the severity of these injuries when mice are exposed to ZnCl2 is much higher than when they are exposed to ZnO NPs, ZnSO4, and Zn (NO3)2.
Keywords
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