Journal of Natural Environment

The Effect of Nitrilo Triacetic Acid (NTA) on Cd Phytoremediation by Maize in Soil Leaching Condition

Document Type : Research Paper

Authors

1 PhD Student, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

2 Professor, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

3 Assistant Professor, Department of Soil Science, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Phytoremediation is a method to refine heavy metals from soil. Using chelators can increase the efficiency of phytoremediation. The aim of this study was to investigate the effect of Nitrilo Triacetic Acid (NTA) on growth indexes and the efficiency of phytoremediation by maize in Cd-contaminated soils under leaching condition. In this way, in the greenhouse, an experiment was conducted consisting three levels of Cd contamination (0, 25 and 50 mgkg-1 soil) and three levels of NTA (0, 15 and 30 mmolL-1 in pot). According to the results, the most amount of Cd in the shoot and root of the plant was observed in Cd50NTA30 (50 mgCd/kg soil and 30 mmolNTA/L in pot) by 2 and 1.8 times, respectively, than Cd50NTA0 (non-application of NTA). Also, the amount of Cd in drainage water in both of Cd25 and Cd50 with apply NTA30 were the maximum amount. In Cd50NTA30 toxicity symptoms were observed in the form of yellowish leaves, decreased chlorophyll index, necrosis and a significant decrease in dry weight of plant organs, leaf area, and plant height. In Cd50NTA30 the amounts of BAC and BCF were increased by 2.8 and 2.4 times, respectively, than Cd50NTA0. In the case of similar treatments, the amount of TI was decreased by 33%. TF could only show a significant increase in Cd50NTA30 than Cd50NTA0. According to the results, the use of NTA as a chelator that has the least environmental damage can significantly increase the efficiency of phytoremediation by maize.

Keywords

Alaboudi, K.A., Ahmed, B., Brodie, G., 2018. Phytoremediation of Pb and Cd contaminated soils by using sunflower (Helianthus annuus) plant. Annals of Agricultural Sciences 63,123–127.
Amin, H., Arain, B.A., Jahangir, T.M., Abbasi, M.S., Amin, F., 2018. Accumulation and distribution of lead (Pb) in plant tissues of guar (Cyamopsis tetragonoloba L.) and sesame (Sesamum indicum L.): profitable phytoremediation with biofuel crops. Geology, Ecology, and Landscapes 2, 51-60.
Anning, A.K., Akoto, R., 2018. Assisted phytoremediation of heavy metal contaminated soil from a mined site with Typha latifolia and Chrysopogon zizanioides. Ecotoxicology and Environmental Safety 148, 97-104.
Bai, W., 2018. Effects of application of NTA and EDTA on accumulation of soil heavy metals in chrysanthemum. IOP Conference Series: Earth and Environmental Science 113, 012175.
Brennan, R.F., Armour, J.D., Reuter, D.J., 1993. Diagnosis of zinc deficiency. In A.D. Robson (Ed.) Zinc in Soils and Plants, P206. Springer, Netherlands. pp. 167-181.
Carter, M.R., Gregorich, E.G., 2008. Soil sampling and methods of analysis (2nd ed). CRC Press. Boca Raton. FL. 1204 p.
Day, P.R., 1982. Particle fractionation and particle-size analysis. In Methods of soil analysis. Page, A.L., R.H. Miller, D.R. Keeney (Ed.). American Society of Agronomy, Madison, Wisconsin. pp: 935-951.
Evangelou, M., Ebel, M., Schaeffer, A., 2007. Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity and fate of chelating agents. Chemosphere 68, 989-1003.
Fotovat, A., 2018. Heavy metals in soils. Alloway, B.J. (Eds.). Ferdowsi University Press, Mashhad. 704 p. (in Persian).
Guo, D., Ali, A., Ren, C., Du, J., Li, R., Lahori, A.H., Xiao, R., Zhang, Z., Zhang, Z., 2019. EDTA and organic acids assisted phytoextraction of Cd and Zn from a smelter contaminated soil by potherb mustard (Brassica juncea, Coss) and evaluation of its bioindicators. Ecotoxicology and Environmental Safety 167, 396-403.
Karimi, R., Chorom, M., Solhi, S., Solhi, M., Safe, A., 2012. Potential of Vicia faba and Brassica arvensis for phytoextraction of soil contaminated with cadmium, lead and nickel. African Journal of Agricultural Research 7, 3293-3301.
Li, X., Zhang, X, Wu, Y., Li, B., Yang, Y. 2018. Physiological and biochemical analysis of mechanisms underlying cadmium tolerance and accumulation in turnip. Plant Diversity 40, 19-27.
Lindsay, W.L., Norvell, W.A., 1979. Development of a DTPA soil test for zinc, iron, manganese, and copper. Soil Science Society America Journal 42, 421-428.
Loppert, R.H., Suarez, D.L., 1996. Carbonate and gypsum. In: D. L. Sparks et al. (Ed.) Methods of Soil Analysis. Part 3, SSSA, ASA, Madison, WI. pp 437-474.
Mananze, S.E., Pocas, I., Cunha, M., 2018. Maize leaf area estimation in different growth stages based on allometric descriptors.  African journal of agricultural research 13, 202-209.
Mertens, J., Vervaeke, P., Meers, E., Tack, F.M.G., 2006. Seasonal changes of metals in willow (Salix sp.) stands for phytoremediation on dredged sediment. Journal of Environmental Science and Technology 40, 1962-1968.
Miller, G., Davis, C., Begonia, G., Begonia, M., 2016. Metal Uptake, Growth Responses, and Chlorophyll Production of Wheat (Triticum aestivum) Exposed at Different Durations to Chelate-Amended Cadmium-Contaminated Soils. World Environment 6, 10-18.
Mulligan, C.N., R. N. Yong, and B. F. Gibbs. 2001. Remediation technologies for metal-contaminated soils and groundwater: An evaluation. Engineering Geology 60, 193-207.
Olsen, S.R., Cole, C.V., Watanabe, F.S., Dean, L.A., 1954. Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA. Circ. 939. U. S. Gov. Print. Office, Washington, DC.
Ozkan, A., Gunkaya, Z., Banar, M., 2016. Pyrolysis of plants after phytoremediation of contaminated soil with lead, cadmium and zinc. Bulletin of Environmental Contamination and Toxicology 96, 415-419.
Sharma, S., Prasad, F.M., 2010. Accumulation of Lead and Cadmium in soil and vegetable crops along major highways in Agra (India).  Electronic Journal of Chemistry 7, 1174-1183.
Soleimani, M., Hajabbasi, M.A., Afyuni, M., Akbar, S., Jensen, J.K., Holm, P.E., Borggaard, O.K. 2010. Comparison of natural humic substances and synthetic ethylenediaminetetraacetic acid and nitrilotriacetic acid as washing agents of a heavy metal polluted soil. Journal of Environmental Quality 39, 855-862.
Song, Y., Ammami, M.T., Benamar, A., Mezazigh, S., Wang, H., 2016. Effect of EDTA, EDDS, NTA and citric acid on electrokinetic remediation of As, Cd, Cr, Cu, Ni, Pb and Zn contaminated dredged marine sediment. Environmental Science and Pollution Research 23, 10577-10586.
Soon, Y.K., Abboud, S., 1993. Cadmium, chromium, lead and nickel. Soil sampling and method of analysis. Lewis Publishers, pp. 103-107.
Sumner, M.E., Miller, W.P., 1996. Cation exchange capacity, and exchange coefficients. In D.L. Sparks (ed.), Methods of soil analysis. p1320. Part 2: Chemical properties, (3rd ed.) ASA, SSSA, CSSA, Madison, WI, pp. 1201-1231.
Tian, S., Jia, Y., Ding, Y., Wang, R., Feng, R., Song, Z., Guo, J., Zhou, L., 2014. Elevated atmospheric CO2 enhances copper uptake in crops and pasture species grown in copper-contaminated soils in a micro-plot study. Clean-Soil, Air, Water 42, 347-354.
Walkley, A., Black, I.A., 1934. Review examination of the degtjareff method determining soil organic matter and a proposed modification of the chromic acid titration method. Journal of Soil Science 34, 29-38.
Wu, J., Hsu, F., Cunningham, S., 1999. Chelate–assisted Pb phytoextraction: Pb availability, uptake and translocation constrains. Environmental Science and Technology 33, 1898-1904.
Yin, Y., Wang, Y., Liu, Y., Zeng, G., Hu, X., Hu, X., Zhou, L., Guo, Y., Li. J,. 2015. Cadmium accumulation and apoplastic and symplastic transport in Boehmeria nivea (L.) Gaudich on cadmium-contaminated soil with the addition of EDTA or NTA. RSC Advances 5, 47584-47591.
Zhang, L., Zhang, L., Song, F., 2008. Cadmium uptake and distribution by different maize genotypes in maturing stag. Communications in Soil Science and Plant Analysis 39, 1517-1531.
Zhao, S., Jia, L., Duo, L., 2016. Combining nitrilotriacetic acid and permeable barriers for enhanced phytoextraction of heavy metals from municipal solid waste compost by lolium perenne and reduced metal leaching. Journal of Environmental Quality 45, 933-939.
Zhou, J.M., Dang, Z., Chen, N.C., Xu, S.G., Xie, Z.Y., 2007. Influence of NTA on accumulation and chemical form of copper and zinc inmaize. Journal of Agro-Environment Science 26, 453-457.
Journal of Natural Environment
Volume 72, Issue 4
December 2019
Pages 499-513