Journal of Natural Environment

Journal of Natural Environment

Comparison of zinc and chromium bioaccumulation in the internal organs of freshwater mussel Anodonta cygnea (Bivalvia: Unionidae)

Document Type : Research Paper

Authors
Fateh Moezzi
Arash Javanshir 1
Soheil Eagderi 2
Hadi Poorbagher 2
1 Associate professor, department of fisheries, faculty of natural resources, university of Tehran, Iran
2 Assistant professor, department of fisheries, faculty of natural resources, university of Tehran, Iran
10.22059/jne.2015.56939
Abstract
Nowadays, bivalves are used in monitoring levels of heavy metals in aquatic environments. The aim of this study was to investigate bioaccumulation of heavy metals zinc (Zn) and chromium (Cr) in the internal organs of Anodonta cygnea. For this purpose, bivalve specimens were collected from Semeskandeh region, Sari, and then in laboratory, were exposed to 125 µg l-1 concentrations of each metal for 27 days. Basal levels, accumulated amounts and ΔC/C0 of both metals in mantle, gill, hepatopancreas and foot of the mussel during study period were measured. Zn basal levels in all studied organs were significantly (P < 0/01) higher than Cr. There were no difference in Zn basal levels between different organs, but for the case of Cr, basal levels were different: gill > hepatopancreas ≈ foot > mantle. Highest accumulation of both metals were observed in gill and hepatopancreas and minimum accumulation were belonged to the mantle. Calculated ratios of ΔC/C0 for Cr were significantly (P < 0/01) higher than Zn in all studied organs: Zn: hepatopancreas > foot > mantle > gill; Cr: mantle > hepatopancreas ≈ foot > gill. Based on our results, it can be said that A. cygnea, is a good species for biomonitoring Zn and Cr levels in aquatic ecosystems.
Keywords
"Aquatic environments"
"Bivalves"
"Heavy metal"
"Bioaccumulation"
  1. Abdullah, M.H., Jovita, S., Ahmad, Z.A., 2007. Heavy metals (Cd, Cu, Cr, Pb and Zn) in Meretrix meritrix roding, water and sediments from estuaries in Sabh, N Bomeo. International journal of environmental and science education, 2(3), 69-74
  2. Abel, P.D., 2002. Water pollution biology. Taylor and Freancis group. New York, USA. 286p
  3. Al-Subiai, Sh.N., Moody, A.J., Mustafa, S.A., Jha, A.N., 2011. A multiple biomarker approach to investigate the effects of copper on the marine bivalve mollusc, Mytilus edulis. Ecotoxicology and environmental safety, 74, 1913-1920
  4. Anandraj, A., Marshall, D.J., Gregory, M.A., McClurg T.P., 2002. Metal accumulation, filtration and O2 uptake rates in the mussel Perna perna (Mollusca: Bivalvia) exposed to Hg2+, Cu2+ and Zn2+. Comparative biochemistry and physiology C: Toxicological pharmacology and endocrinology, 132, 355-363
  5. Azarbad, H., Javanshir, A., Daneh-kar, A., Shapouri, M., 2010. Biosorption and bioaccumulation of heavy metals by rock oyster Saccostrea cucullata in the Persian Golf. International aquatic research, 2, 61-69
  6. Banfalvi, G., 2011. Cellular effects of heavy metals. Springer, London, U.K., 348p
  7. Bowen, H.J.M., 1976. Trace elements in biochemistry. Academic press, New York, USA. 387p
  8. Bryan, G.W., 1973. The occurrence and seasonal variation of trace metals in the scallops Pecten maximus (L.) and Chlamys opercularis (L.). Journal of marine biological association U.K., 53, 145-166
  9. Buhl, K.J., Hamilton, S.J., 1990. Comparative toxicity of inorganic contaminants related by placer mining to early life stages of salmonids. Ecotoxicology and environmental safety, 20, 325-342
  10. Burry, N.R., Walker, P.A., Glover, C.N., 2003. Nutritive metal uptake in teleost fish. Journal of experimental biology, 206(1), 11-33
  11. Bustamante, P., Miramand, P., 2005. Subcellular and body distributions of 17 trace elements in the variegated scallop Chlamys varia from the French coast of the Bay of Biscay. Science of total environment, 337, 59-73
  12. Cardoso, P.G., Lillebo, A.I., Pereira, E., Duarte, A.C., Pardal, M.A., 2009. Different mercury bioaccumulation kinetics by two macrobenthic species: The bivalve Scrobicularia plana and the polychate Hediste diversicolor. Marine environmental research, 68, 12-18
  13. Chavez-Crooker, P., Garrido, N., Pozo, P., Aheam, G.A., 2003. Copper transport by lobster (Homarus americanus) hepatopancreatic lysosomes. Comparative biochemistry and physiology, 135(2), 107-118
  14. Choi, H.J., Ji, J., Chung, K-H., Ahn, I-Y., 2007. Cadmium bioaccumulation and detoxification in the gill and digestive gland of the Antarctic bivalve Laternula elliptica, Comparative biochemistry and physiology, Part C, 145, 227-235
  15. Coombs, T.L., 1972. The distribution of zinc in the oyster Ostrea edulis and its relation with enzymatic activity and to other metals. Marine biology, 12, 170-178
  16. Deforest, D.K., Brix, K.V., Adams, W.J., 2007. Assessing metal bioaccumulation in aquatic environments: the inverse relationship between bioaccumulation factors, trophic transfer factors and exposure concentration. Aquatic toxicology, 84, 236-246
  17. Ernst, G., Frey, B., 2007. The effect of feeding behaviour on Hg accumulation in the ecophysiologically different earthworms Lumbricus terrestris and Octolaseon cyaneum: a microcosm experiment. Soil biology and biochemistry, 30, 386-390
  18. Farris, J.L., Van Hassel, J.H., 2007. Freshwater bivalve Ecotoxicology. CRC Press, Taylor and Francis group, New York, USA. 375p
  19. Forstner, U., Wittman, G.T., 1983. Marine pollution in the aquatic environment. Springer, London, U.K. 376P
  20. Fournier, M., Pellerin, J., Clermont, Y., Morin, Y., Brousseau, P., 2001. Effects of in vivo exposure of Mya arenaria to organic and inorganic mercury on phagocytic activity of haemocytes. Toxicology, 161, 210-211
  21. Fukunaga, A., Anderson, M.J., 2011. Bioaccumulation of copper, lead, zinc by the bivalve Macomona liliana and Austrovenus stutchburyi. Journal of experimental marine biology and biology, 396, 244-252
  22. George, S.G., Pirie, B.J.S., Coombs, T.L., 1980. Isolation and elemental analysis of metal-rich granules from kidney of the scallope Pecten maximus (L.). Journal of marine biology and ecology, 42, 143-156
  23. Golovanova, I.L., Frolova, T.V., 2005. Influence of copper, zinc and cadmium upon carbohydrase activities in aquatic invertebrates. Biologica Vnutrennih Vod., 4, 73-83
  24. Hedouin, L., Pringault, O., Bustamante, P., Fichez, R., Warnau, M., 2011. Validation of two tropical marine bivalves as bioindicators of mining contamination in the New Caledonia lagoon: Field transplantation experiment. Water research, 45, 483-496
  25. Kadar, E., Santos, R.S., Powell, J.J., 2006. Biological factors influencing tissue compartmentalization of trace metals in the deep-sea hydrothermal vent bivalve Bathymodiolus azoricus at geochemically distinct vent sites of the Mid-Atlantic Ridge, Environmental Research, 101, 221-229
  26. Langard, S., Norseth, T., 1979. Chromium. In: Friberg, L., Nordberg, G.F., Vouk, V.B. (eds.), Handbook on the toxicology of metals. Elsevier/North Holland Biomedical Press, pp. 383-397
  27. Liobet, J.M., Domingo, J.L., Colomina, M.T., Mayayo, E., Corbella, J., 1988. Subchronic oral toxicity of zinc in rats. Bulletin of environmental contamination and toxicology, 41, 36-43
  28. Livingstone, D.R., Chipman, J.K., Lowe, D.M., Minier, C., Mitchelmore, C.L., Moore, M.N., Peters, L.D., Pipe, R.K., 2000. Development of biomarkers to detect the effects of organic pollution on aquatic invertebrates: recent immunological studies on the common mussel (Mytilus edulis L.) and other mytilids. International journal of Environmental Pollution, 13, 1-6
  29. Mauri, M., Orlando, E., Nigro, M., Regoli, F., 1990. Heavy metal in the Antarctic scallop Adamussium colbecki. Marine ecology progress series, 67, 27-33
  30. Metian, M., Bustamante, P., Hedouin, L., Oberhansli, F., Warnau, M., 2009. Delineation of heavy metal uptake pathways (seawater and food) in the variegated scallop Chlamys varia using radiotracer techniques. Marine ecology progress series, 375, 161-171
  31. Moloukhia, H., Sleem, S., 2011. Bioaccumulation, fate and toxicity of two heavy metals common in industrial wastes in two aquatic molluscs. Journal of American science, 7(8), 459-464.
  32. Naimo, T.J., 1995. A review of the effects of heavy metals on freshwater mussels. Ecotoxicology, 4, 341-362
  33. Paez-Osuna, F., Ochoa-Izaguirre, M.J., Bojorquez-Leyva, H., Michel-Reynoso, I.L., 2000. Macroalgae as biomonitors for metal availability in coastal lagoons from the subtropical Pacific of Mexico. Bulletin of environmental contamination and toxicology, 64(6), 846-851
  34. Pawlisz, A.V., Kent, R.A., Schneider, U.A., Jefferson, C., 1997. Canadian water quality guidelines for chromium. Environmental toxicology and water quality, 12, 185-193
  35. Pecquenant, J.E., Fowler, S.W., Small, L.F., 1969. Estimates of the zinc requirements of marine organisms. Journal of fisheries research board of Canada, 26, 145-150
  36. Pourang, N., Richardson, C.A., Mortazavi, M.S., 2010. Heavy metal concentration in the soft tissues of swan mussel (Anodonta cygnea) and surficial sediments from Anzali wetland, Iran. Environmental monitoring and assessment, 163, 195-213
  37. Rainbow, P.S., 2002. Trace metal concentrations in aquatic invertebrates: why and so what? Environmental Pollution, 120, 497-507
  38. Regoli, F., Principato, G., 1995. Glutathione, glutathione-dependent and antioxidant enzymes in mussel, Mytilus galoprovincinalis, exposed to metals under field and laboratory conditions: implications for the use of biochemical biomarkers. Aquatic toxicology, 31, 143-164
  39. Scaps, P., 2002. A review of the biology, ecology and potential use of the common ragworm Hediste diversicolor (O.F. Muller) (Annelida: Polychaeta). Hydrobiologia, 470, 203-218
  40. Sanders, B.M., 1993. Stress proteins in aquatic organisms: an environmental perspective. Critical Review of Toxicology, 23(1), 49-75
  41. Simkiss, K., 1981. Cellular discrimination processes in metal accumulating cells. Journal of experimental biology, 94, 317-327
  42. Stewart, A.R., 1999. Accumulation of Cd by a freshwater mussel (Pyganodon grandis) is reduced in the presence of Cu, Zn, Pb, and Ni. Canadian journal of fisheries and aquatic science, 56, 467-478
  43. Towill, L.E., Shriner, C.R., Drury, J.S., Hammons, A.S., Holleman, J.W., 1978. Reviews of the environmental effects of pollutants: III chromium. U.S. environmental protection Agency Rep., 600/1-78-023. 287P
  44. Van Duren, L.A., Herman, P.M.J, Sandee, A.J.J., Heip, C.H.R., 2006. Effects of muscle filtering activity on boundary layer structure. Journal of Sea Research, 55, 3-14
  45. Viarengo, A., Zanicchi, G., Moore, M.N., Orunesu, M., 1981. Accumulation and detoxification of copper by the mussel Mytilus galoprovincialis Lam: a study of the subcellular distribution in the digestive gland cells. Aquatic toxicology, 1, 147-157
  46. Wang, W.-Xi., Rainbow, P.S., 2008. Comparative approaches to understand metal bioaccumulation in aquatic animals. Comparative biochemistry and physiology, Part C, 148: 315-323
  47. Waykar, B., Deshmukh, G., 2012. Evaluation of bivalves as bioindicators of metal pollution in freshwater. Bulletin of environmental contamination and toxicology, 88, 48-53
  48. WHO. 2001. Environmental health criteria: Zinc. Geneva. 123p
  49. Williams, R.J.P., 1981. Physic-chemical aspects of inorganic element transfer through membranes. Philosophical transactions of the royal society B: Biological sciences, 294, 57-74.
Journal of Natural Environment
Volume 68, Issue 4 - Serial Number 4
Winter 2016
Pages 677-686