بررسی تأثیر کاربرد ضایعات آلی و تلقیح باکتری رودوکوکوس در کاهش هیدروکربن‌های نفتی کل یک خاک شور آلوده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم و مهندسی خاک، دانشکده مهندسی و فناوری کشاورزی، دانشگاه تهران، کرج، ایران

2 پژوهشکده توسعه صنایع شیمیایی جهاد دانشگاهی، تهران، ایران

3 گروه مهندسی ماشین‌‌های کشاورزی، دانشکده مهندسی و فناوری کشاورزی، دانشگاه تهران، ایران

چکیده

پاکسازی آلودگی نفتی در محیط ناهمگون خاک با پیچیدگی ­هایی روبه­رو است که عواملی نظیر شوری خاک و سن آلودگی به ­سختی آن می ­افزایند. این پژوهش با هدف ارزیابی تأثیر یکی از سویه ­های بومی باکتری رودوکوکوس و انواع پسماندهای آلی در کاهش هیدروکربن‍‍ های نفتی کل یک خاک شور آلوده به نفت خام در شرایط انکوباسیون اجرا شد. بدین منظور، آزمایشی به ­صورت فاکتوریل کامل در قالب طرح کاملاً تصادفی با چهار فاکتور تلقیح باکتریاییRhodococcus cercidiphylli Y1M65003 ، پنج‌درصد ضایعات کمپوست قارچ، پنج‌درصد باگاس نیشکر و افزودن منبع نیتروژن و فسفر با نسبت 100:10:1 از منبع اوره و سوپرفسفات تریپل طراحی گردید. نتایج تجزیه واریانس داده ­های حذف هیدروکربن ­های کل نشان داد که چهار فاکتور مورد بررسی توانستند اثر معنی­ داری (0/001>P) بر کاهش هیدروکربن­ های کل خاک داشته باشند که از میان آن‌ها فاکتور تلقیح باکتریایی سهم بیشتری در واریانس متغیر وابسته داشت. اثرات متقابل مایة تلقیح باکتریایی با هر یک از فاکتورهای ضایعات کمپوست قارچ، باگاس و منبع نیتروژن و فسفر نیز بر تجزیۀ هیدروکربن‍ های کل خاک معنی ­دار بود (0/01>P). کاراترین تیمار در کاهش هیدروکربن ­های کل نفت خاک تلفیقی از فاکتورهای مایه تلقیح باکتریایی، ضایعات کمپوست قارچ و منبع نیتروژن و فسفر بود. این تیمار توانست در طی 60 روز، 35/4درصد از هیدروکربن­ های کل نفت خاک را کاهش دهد. نتایج نشان داد که افزودن ضایعات کمپوست قارچ و تحریک زیستی ناشی از منبع نیتروژن و فسفر می­ تواند توان باکتری رودوکوکوس را در تجزیۀ هیدروکربن ­های نفتی افزایش دهد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of utilizing organic waste and Rhodococcus bacterium in reducing the total petroleum hydrocarbons of a contaminated saline soil

نویسندگان [English]

  • Hanye Jafari Vafa 1
  • Ahmad Ali Pourbabaee 1
  • Hossein Ali Alikhani 1
  • Najmeh Yazdanfar 2
  • Majid Khanali 3

1 Department of Soil Science and Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran, Karaj, Iran

2 Iranian Institute of R&D in Chemical Industries (ACECR), Tehran, Iran

3 Department of Agricultural Machinery Engineering, Faculty of Agricultural Engineering and Technology, University of Tehran< Iran

چکیده [English]

There are hardships in oil-polluted soil remediation that are increased by factors, such as soil salinity and pollution age. This study aimed to evaluate the effect of the native strain of Rhodococcus bacterium and organic waste on the the total petroleum hydrocarbons reduction in contaminated saline soil by crude oil in incubation condition. For this purpose, a full factorial experiment using a completely randomized design was conducted with four factors: bacterial inoculation of Rhodococcus cercidiphylli Y1M65003, 5% spent mushroom compost, 5% sugarcane bagasse, and addition nitrogen and phosphorus with 100: 10: 1 ratio from urea and superphosphate triple. The analysis of variance of total petroleum hydrocarbons elimination showed that all four factors could significantly affect (p

کلیدواژه‌ها [English]

  • Spent mushroom compost
  • Bagasse
  • Petroleum pollution
  • Biostimulation
  • Bioaugmentation
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