Work culture based on Islamic perspective in organizations is one of the most important ways to redeem the countries from economic problems, especially underdeveloped countries. This study aimed to investigate the status of work culture based on Islamic perspective in the country's environmental organization. In this descriptive-survey study, a total number of 400 experts familiar with the theories of work culture based on Islamic perspective and employees with high organizational rank (in 31 provinces) were selected according to Cochran's formula. A questionnaire was therefore used to collect data. For data analysis, descriptive and inferential statistics (Pearson correlation, univariate t-test, Kolmogorov-Smirnov test, and exploratory and confirmatory factor analysis) were applied. Dimension and component indicators of Islamic work culture proposed by experts include Individual factors (need for success, sense of independence and desire for power), Organizational (customer orientation, financial resource management and maintaining the position of the organization), Social (participation and teamwork, employee trust and well-being), Environmental (achievement of functional goals, creativity and political intervention) and Technological (thinking and analysis, staff training methods and communication skills). According to the friedman test,"Organizational factors" and "Maintaining the position of the organization" were at the first place. In the meantime, tWork culture based on Islamic perspective and its dimensions were in a desired condition.